Quotations are hereby invited for the Supply of the following: • PCR plate sealing film, aluminum, 35um 100/pkt x 4 Each: PCR plate sealing film, aluminum, 35um 100/pkt pierceable, non sterile. The above specified goods should be delivered to: Name of Institute: ARC-OVI: Address: 100 Old Soutpan Road (M35): Onderstepoordt: Private Bag X05, 0110. Please note that this RFQ was published late. (Тендер №72704025ru)

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Язык: EN
Номер: 72704025
Дата публикации: 03-11-2022
Источник: ONLINE TENDERS

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Quotations are hereby invited for the Supply of the following: • Quick-DNA Tissue/Insect Miniprep Kit, 50 reactions x 1 Each: - Format - Bead Beating, Spin Column. - Sample Sources - Samples (n = 1 and = 50 mg) of fresh, frozen, or stored insects including: mosquitoes, bees, lice, ticks, D. melanogaster, etc. Also, compatible with fresh or frozen mammalian tissues as well as cultured cells, and whole blood; - DNA Yield - Expected yields can range from 1 - 5 µg DNA per mg specimen sampled. For mammalian tissues, yields are 1 - 3 µg DNA per mg of skeletal, heart and brain tissues and 3 - 5 µg DNA per mg of liver, kidney, and lung tissues. Whole blood will yield from 3 - 7 µg DNA per 100 µl; - DNA Purity - High quality DNA is eluted with DNA Elution Buffer that is well suited for PCR amplification, endonuclease digestion, etc. A260/A280 >1.8; - DNA Size Limits - Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. - DNA Recovery - Typically, up to 25 µg total DNA is eluted into 100 µl (35 µl minimum) DNA Elution. The above specified goods should be delivered to: Name of Institute: ARC-OVI: Address: 100 Old Soutpan Road (M35): Onderstepoordt: Private Bag X05, 0110. Please note that this RFQ was published late. Источник: ONLINE TENDERS

Quotations are hereby invited for the Supply of the following: • Quick-DNA Tissue/Insect Miniprep Kit, 50 reactions x 1 Each: - Format - Bead Beating, Spin Column. - Sample Sources - Samples (n = 1 and = 50 mg) of fresh, frozen, or stored insects including: mosquitoes, bees, lice, ticks, D. melanogaster, etc. Also, compatible with fresh or frozen mammalian tissues as well as cultured cells, and whole blood; - DNA Yield - Expected yields can range from 1 - 5 µg DNA per mg specimen sampled. For mammalian tissues, yields are 1 - 3 µg DNA per mg of skeletal, heart and brain tissues and 3 - 5 µg DNA per mg of liver, kidney, and lung tissues. Whole blood will yield from 3 - 7 µg DNA per 100 µl; - DNA Purity - High quality DNA is eluted with DNA Elution Buffer that is well suited for PCR amplification, endonuclease digestion, etc. A260/A280 >1.8; - DNA Size Limits - Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. - DNA Recovery - Typically, up to 25 µg total DNA is eluted into 100 µl (35 µl minimum) DNA Elution. The above specified goods should be delivered to: Name of Institute: ARC-OVI: Address: 100 Old Soutpan Road (M35): Onderstepoordt: Private Bag X05, 0110. Please note that this RFQ was published late. Источник: ONLINE TENDERS

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Quotations are hereby invited for the Supply of the following: • Taq DNA Polymerase, 1000u x 1 Each: - 1000u, Concentration: 5 units/µl; - Recombinant enzyme: Yes; - Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11- - dUTP, fluorescent-dNTP/ddNTP; - Extension rate: 2–4 kb/min at 72°C; - Half-life: 10 min at 97°C; 60 min at 94°C; - Amplification efficiency: =105 fold; - 5" – > 3" exonuclease activity: Yes; - Extra A addition: Yes; - 3" – > 5" exonuclease activity: No; - Contaminating nucleases: No; - Contaminating R Nases: No; - Contaminating proteases: No; - Self-priming activity: No. The above specified goods should be delivered to: Name of Institute: ARC-OVI: Address: 100 Old Soutpan Road (M35): Onderstepoordt: Private Bag X05, 0110. Please note that this RFQ was published late. Источник: ONLINE TENDERS

Quotations are hereby invited for the Supply of the following: • Taq DNA Polymerase, 1000u x 1 Each: - 1000u, Concentration: 5 units/µl; - Recombinant enzyme: Yes; - Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11- - dUTP, fluorescent-dNTP/ddNTP; - Extension rate: 2–4 kb/min at 72°C; - Half-life: 10 min at 97°C; 60 min at 94°C; - Amplification efficiency: =105 fold; - 5" – > 3" exonuclease activity: Yes; - Extra A addition: Yes; - 3" – > 5" exonuclease activity: No; - Contaminating nucleases: No; - Contaminating R Nases: No; - Contaminating proteases: No; - Self-priming activity: No. The above specified goods should be delivered to: Name of Institute: ARC-OVI: Address: 100 Old Soutpan Road (M35): Onderstepoordt: Private Bag X05, 0110. Please note that this RFQ was published late. Источник: ONLINE TENDERS