Quotations are hereby invited for the following: Supply of LR One-step qRT PCR kit. Scope: 1. One step RT-PCR kit (100 rxns or more): One-Step Reverse Transcription-PCR System combining Reverse Transcriptase (RT) and high-fidelity DNA Polymerase to provide superior one-step RT-PCR performance. One-Step RT-PCR System that is designed for sensitive end-point detection and analysis of RNA by RT-PCR. The convenient formulation enables both cDNA synthesis and PCR amplification to be performed in a single reaction tube using gene-specific primers. Be able to amplify RNA targets up to 14 kb in length, and is compatible with multiplex RT-PCR. Allows high efficiency cDNA synthesis due to high thermostability, processivity, and have the ability to synthesize cDNA efficiently from a variety of RNA samples. The mix should contain a DNA Polymerase which has high specificity, offers high yield, and is ideally suited for PCR applications that require sequence accuracy. Enzyme must be MMLV or MMLV derivative. Minimum 100rxns per kit, Qty: 03. 2. One step qRT-PCR its (100 rxns or more): One-Step real-time Reverse Transcription-PCR System combining Reverse Transcriptase (RT) and high fidelity DNA Polymerase to provide superior one-step real time RT-PCR performance. One-Step qRT-PCR System that is designed for sensitive end-point detection and analysis of RNA by real-time RT-PCR. The convenient formulation enables both cDNA synthesis and PCR amplification to be performed in a single reaction tube using gene-specific primers. Be able to amplify RNA targets up to 14 kb in length, and is compatible with multiplex RT-PCR. Allows high efficiency cDNA synthesis due to high thermostability, processivity, and have the ability to synthesize cDNA efficiently from a variety of RNA samples. The mix should contain a DNA Polymerase which has high specificity, offers high yield, and is ideally suited for PCR applications that require sequence accuracy. Enzyme must be MMLV or MMLV derivative. 100 or more rxn per kit, Qty: 04. 3. First strand cDNA synthesis kit (50 rxns or more First-Strand Synthesis System for RTPCR optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA). RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. Enzyme must be MMLV or MMLV derivative. 50 rxns or more per kit, Qty: 01. 4. Second strand cDNA synthesis kit (50 rxns or more). For the synthesis of second strand cDNA compatible with Illumina down stream sequencing. 50rxns or more, Qty: 01. Address: ARC-Onderstepoort Veterinary Institute (ARC-OVI); 100 Old Soutpan Road (M35); Private Bag X05; Onderstepoort; 0110. (Тендер №77515195ru)